目的 筛选不同叶色部位稳定表达的内参基因，为后期开展‘麦缘锦楸’叶色形成的分子机制奠定基础。 方法 以‘麦缘锦楸’不同叶色部位及灰楸对应部位叶片为材料，利用实时荧光定量PCR（qRT-PCR）技术检测了7个候选基因（CfUBC、CfActin11、CfPP2A、CfMADH、CfGADPH、CfEF-1及CbuActin）的相对表达量，利用GeNorm、NormFinder和BestKeeper 等分析软件对内参基因进行了稳定性评价。分析了‘麦缘锦楸’和灰楸不同叶色部位中萜类合成相关基因（CfGES）的表达模式，验证了上述稳定性评价结果的可靠性。 结果 7个候选内参基因在‘麦缘锦楸’和灰楸叶片中均具有作为内参基因的性能，其中，CfMADH和CfEF-1在不同叶色部位的表达量最稳定，CfGADPH和CfActin11次之，CfUBC最差。以萜类合成相关基因CfGES验证内参稳定性发现，单独或组合使用CfEF-1及CfMADH为内参基因时，CfGES的表达差异与转录组数据趋势一致。 结论 单独或组合使用CfMADH和CfEF-1来校准‘麦缘锦楸’不同叶色部位的基因表达量，可显著提高实验结果的可靠性。

本文以改进CTAB法提取的总RNA为模板,通过设计茎环反转录引物,利用定量RT-PCR技术从黑杨幼嫩叶片、成熟叶片中均检测到miRNA。结果表明,miR159、miR167、miR171、miR319在三倍体幼叶中表达量均高于二倍体,miR164在三倍体成熟叶片中的表达量高于二倍体表达量。除miR319外,其余miRNA与其相应的靶基因在二倍体和三倍体黑杨不同阶段叶片中表达的消长关系都较为典型。暗示这些miRNA 靶基因可能与黑杨三倍体的速生性状形成相关。

Paulownia wood is of great quality, but the wood stain is easily occurred after sawing. 29 Paulownia fortunei trees which have excellent growing properties were selected by properties of wood color, 5 indexes were used to compare and analyze the wood color, including psychometric lightness, red degree, yellow degree, color difference and Hunter whiteness. The result shows that the differences of each index among individuals reach to an extremely significant level. It is concluded that certain potentiality exists in excellent selection from individuals. The Cluster analysis of all individuals with the 5 indexes was did by median method, 5 individuals which prior clustered together: superior fortunei 4, superior fortunei 25, superior fortunei 5, superior fortunei 3 and superior fortunei 2 were selected, the selection rate is 17.24%. The Hunter whiteness and psychometric lightness values of individuals selected were significantly higher than other clusters, the color difference were significantly lower than other clusters, the red degree and yellow degree were in the middle, the average Hunter whiteness values of individuals selected reached to 75.060, the selection differential was 3.818, selection intensity was 1.297. These individuals have excellent color properties, superior stem form and no disease, can be widely applied in the production.

以"毛泡桐×白花泡桐"的优良无性系TF33和两个标准对照品种(白花泡桐C001与兰考泡桐C125)为试验材料, 研究了泡桐优良无性系干材表型性状的杂种优势。结果表明:(1)TF33、C001、C125无性系间各性状存在广泛和显著的变异;(2)3个生长性状(接干高、接干材积和主干材积)与3个干形性状(通直度、接干形数和主干平均削度)为高度变异性状(CV≥30%), CV变幅为33.10%53.19%;(3)接干因子是影响和决定性状杂种优势最终状况和总体表现的最重要因素;(4)通直度性状的遗传改良是毛泡桐×白花泡桐杂种无性系干形遗传改良的关键和瓶颈;(5)多数性状间存在极显著或显著的相关性, 且不同的性状间相关性变化较大;(6) TF33较C001有显著的材积生长杂种优势, 较C125没有显著杂种优势。